Novel mouse model for carcinoembryonic antigen-based therapy

A novel transgenic mouse model for immunological evaluation of carcinoembryonic antigen-based DNA minigene vaccines.

A lack of relevant animal models has hampered preclinical screening and critical evaluation of the efficacy of human vaccines in vivo. Carcinoembryonic antigen-A2Kb (CEA-A2Kb) double transgenic mice provide a biologically relevant model for preclinical screening and critical evaluation of human CEA vaccine efficacy in vivo, particularly because such animals are peripherally tolerant of CEA. We ...

Strategies for cancer therapy using carcinoembryonic antigen vaccines.

Advances in molecular biology and immunology have renewed interest in the development of vaccines for the treatment or prevention of cancer. Research over the past 10 years has focused on the identification of suitable tumour antigens to use as targets for a variety of vaccine strategies. Carcinoembryonic antigen (CEA) was one of the first tumour antigens described, and is commonly expressed by...

Enzyme immunoassay for carcinoembryonic antigen.

In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the pe...

An aptasensor based on electrosynthesized conducting polymers, Cu2O–carbon dots and biosynthesized gold nanoparticles, for monitoring carcinoembryonic antigen

Current work proposes an inimitable composite, with great electrical conductivity and quite enhanced surface area, (including conducting polymers (poly (cathechol)), Cu2O–carbon dots and green synthesized gold nanoparticles) for detecting acute carcinoembryonic antigen. At current work, the electropolymerization was offered instead of enzyme-catalyzed polymerization of poly (catechol). <b...

Gene therapy strategies utilizing carcinoembryonic antigen as a tumor associated antigen for vaccination against solid malignancies